NGS

Subsampling a fastq file with awk

What you are going to find here A minimal introduction of the awk command in Linux and Mac (For Mac user, installing GNU awk might be necessary. It introduced some new functions like sorting an array with asort().

Using Limma to find differentially expressed genes

Ritchie, ME, Phipson, B, Wu, D, Hu, Y, Law, CW, Shi, W, and Smyth, GK (2015). limma powers differential expression analyses for RNA-sequencing and microarray studies.Nucleic Acids Research 43(7), e47.

Wandering into next-generation seqeuncing

No Longer That “Next” Generation When I was doing my undergraduate project, microarray was like black magic that turned the labyrinth of gene expression into colorful heatmap and brought your paper into top-notch journals.